Technical skills

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  1. Gene expression analysis including qPCR and RT PCR
  2. DNA and RNA purification
  3. Nuclei isolation
  4. CRISPR Cas9 gene editing
  5. Site directed mutagenesis
  6. Vector and primer design
  7. Transduction, transfection, and chemical and electroporation based transformation
  8. Stable cell line development including HEK293T, CHO, iPSC CM, iPSC, T cell isolation, primary cell lines and transgenic mouse models
  9. AAV, lentivirus, and adenovirus production, titration, and delivery in vitro and in vivo
  10. BSL 2 workflows and aseptic techniques
  1. Protein expression and purification using SEC, HIC, AEX, CEX, and affinity chromatography
  2. Experience purifying more than 100 recombinant proteins
  3. Protein engineering and functional optimization
  4. High throughput screening in 96 and 384 well formats
  5. ELISA and antigen antibody screening
  6. Enzyme kinetics and ligand binding assays (Differential Scanning Fluorimetry (DSF), Differential Scanning Calorimetry (DSC))
  7.  LC/MS
  8. Immunoprecipitation
  9. FRET assays
  10. UV visible spectroscopy

    Instrumentation     includes FPLC with UNICORN, HPLC Agilent systems, UF DF at 50 L scale, TFF, spin and gravity columns, LC MS, SPR, BLI, and DLS
  1. Single cell and single nucleus RNA sequencing
  2. Spatial Transcriptomics
  3. ATAC seq, Multiome, and ChIP seq
  4. 10X Genomics platforms
  5. Library preparation and sequencing workflows
  6. PacBio HiFi sequencing
  7. Gene regulatory network analysis

    Tools and software     include Seurat, Scanpy, Scenic Plus, Signac, Bowtie, pycisTopic, pycisTarget, CellChat, Slingshot, Monocle3, GSEA, CellRanger, Samtools, Bamtools, SnapGene, Schrödinger, and GraphPad Prism

    Sequencing platforms     include Sanger sequencing and Nanopore, Illumina, Pac-bio, Hi-seq, Amplicon sequencing, Barcode sequencing

    Variant analysis and BLAST

  1. Rolling circle amplification (RCA)
  2. In vitro transcription
  3. RCA workflow optimization
  4. dsRNA
  5. High-Throughput Screening

    Instrumentation :     Mya4

  6. Cloning and PCR Technologies: 
    TOPO TA cloning
    Gateway cloning
    T4 ligation
    PCR based cloning techniques
    In Fusion cloning

  1. Immunofluorescence assays
  2. Immunohistochemistry and H and E staining
  3. In Situ Hybridization (ISH)
  4. RNAscope
  5. Probe based/ Reporter assay design
  6. Histology, paraffin embedding, cryosectioning, staining
  7. Masson’s Trichrome staining
  8. Rodent and Tissue handling
  9. Cytokine profiling
  10. Flow cytometry and cell sorting
  11. Cell based labeling assays
  12. Mouse handling and genotyping
  13. Intraperitoneal and viral injections
  14. Blood and tissue collection
  15. MSD, ELISA
  16. Gene silencing and overexpression
  17. shRNA, siRNA
  1. ImageXpress Pico system
  2. Olympus FV3000 resonant scanning confocal microscope
  3. Qulitative and Quantitative analysis
  4. ImageJ/ Fiji
  1. SDS PAGE
  2. Urea acrylamide gels
  3. Western blot
  4. Agarose gel electrophoresis
  5. Dot blot
  6. Capillary electrophoresis
  7. Tapestation
  8. Fragment Analyzer
  1. Protein Engineering,  Protein MPNN, Thermo MPNN, Machine Learning approaches
  2. Alpha Fold, Rosetta, Pymol, Schrödinger
  3. Python
  4. R
  5. Design of Experiments (DoE) : JMP
  6. SPSS
  7. ANOVA based statistical analysis
  8. Operating Systems: Linux, Windows, Mac
  9. Use of Artificial Intelligence (AI) and AI related tools
  10. Use of High Performance Computing (HPC)
  1. GLP and cGMP environments
  2. Technology transfer
  3. Batch record documentation
  4. SOP development and compliance
  5. ISO14001
  6. EHS – Environmental and Health Safety Regulations
  1. Public speaking
  2. Creative problem solving
  3. Critical thinking
  4. Mentorship and team development
  5. Cross functional collaboration
  6. Scientific leadership