Technical skills

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  1. Gene expression analysis including qPCR and RT PCR
  2. DNA and RNA purification
  3. Nuclei isolation
  4. CRISPR Cas9 gene editing
  5. Site directed mutagenesis
  6. Vector and primer design
  7. Transduction, transfection, and chemical and electroporation based transformation
  8. Stable cell line development including HEK293T, CHO, iPSC CM, iPSC, primary cell lines and transgenic mouse models
  9. AAV, lentivirus, and adenovirus production, titration, and delivery in vitro and in vivo
  10. BSL 2 workflows and aseptic techniques
  1. Protein expression and purification using SEC, HIC, AEX, CEX, and affinity chromatography
  2. Experience purifying more than 100 recombinant proteins
  3. Protein engineering and functional optimization
  4. High throughput screening in 96 and 384 well formats
  5. ELISA and antigen antibody screening
  6. Enzyme kinetics and ligand binding assays
  7. Thermal stability and biophysical characterization
  8. Immunoprecipitation
  9. FRET assays
  10. UV visible spectroscopy

    Instrumentation     includes FPLC with UNICORN, HPLC Agilent systems, UF DF at 50 L scale, TFF, spin and gravity columns, LC MS, SPR, BLI, and DLS
  1. Single cell and single nucleus RNA sequencing
  2. ATAC seq, Multiome, and ChIP seq
  3. 10X Genomics platforms
  4. Library preparation and sequencing workflows
  5. Gene regulatory network analysis

    Tools and software     include Seurat, Scanpy, Scenic Plus, Signac, Bowtie, pycisTopic, pycisTarget, CellChat, Slingshot, Monocle3, GSEA, CellRanger, SnapGene, Schrödinger, and GraphPad Prism

    Sequencing platforms     include Sanger sequencing and Nanopore, Hi-seq, Amplicon sequencing, Barcode sequencing

    Variant analysis and BLAST

  1. Rolling circle amplification (RCA)
  2. In vitro transcription
  3. RCA workflow optimization


    Instrumentation :     Mya4

  4. Cloning and PCR Technologies: 
    TOPO TA cloning
    Gateway cloning
    T4 ligation
    PCR based cloning techniques
    In Fusion cloning

  1. Immunofluorescence assays
  2. Immunohistochemistry and H and E staining
  3. Masson’s Trichrome staining
  4. Cytokine profiling
  5. Flow cytometry and cell sorting
  6. Cell based labeling assays
  7. Mouse handling and genotyping
  8. Intraperitoneal and viral injections
  9. Blood and tissue collection
  10. MSD, ELISA
  1. ImageXpress Pico system
  2. Olympus FV3000 resonant scanning confocal microscope
  1. SDS PAGE
  2. Urea acrylamide gels
  3. Western blot
  4. Agarose gel electrophoresis
  5. Dot blot
  6. Capillary electrophoresis
  7. Tapestation
  8. Fragment Analyzer
  1. Python
  2. R
  3. Design of Experiments (DoE) : JMP
  4. SPSS
  5. ANOVA based statistical analysis
  6. Operating Systems: Linux, Windows, Mac
  7. Use of Artificial Intelligence (AI) and AI related tools
  1. GLP and cGMP environments
  2. Technology transfer
  3. Batch record documentation
  4. SOP development and compliance
  5. ISO14001
  6. EHS – Environmental and Health Safety Regulations
  1. Public speaking
  2. Creative problem solving
  3. Critical thinking
  4. Mentorship and team development
  5. Cross functional collaboration
  6. Scientific leadership